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In Situ HCR v3.0
State-of-the-art mRNA imaging:
- Simple: 2-stage enzyme-free protocol
- Amplified: Signal elevated above autofluorescence
- Multiplexed: Simultaneous 1-stage amplification for up to 5 targets
- Quantitative: Signal scales linearly with target abundance
- Penetrating: Whole-mount vertebrate embryos and thick tissue sections
- Resolved: Subcellular or single-molecule resolution as desired
- Sensitive: Single molecules detected in thick autofluorescent samples
- Versatile: Suitable for use with diverse targets in diverse organisms
- Compatible: Combine with tissue clearing as desired
- Robust: Automatic background suppression throughout the protocol
Multiplexed mRNA imaging in a whole-mount chicken embryo using in situ HCR v3.0 without probe set optimization ( Choi et al., 2018).
In situ HCR supports four multiplexed quantitative analysis modes:
- qHCR imaging: Analog mRNA relative quantitation with subcellular resolution in an anatomical context
- dHCR imaging: Digital mRNA absolute quantitation via single-molecule imaging in an anatomical context
- qHCR flow cytometry: Analog mRNA relative quantitation for high-throughput expression profiling of mammalian and bacterial cells
- qHCR northern blots: Relative and absolute quantitation of RNA target size and abundance
In Situ HCR v2.0 Image Gallery
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