In Situ HCR v3.0 Protocol Summary
![]() In situ HCR v3.0: multiplexed quantitative mRNA imaging in thick autofluorescent samples with
automatic background suppression throughout the protocol (Choi et al., 2018). ![]() Multiplexing timeline: the same two-stage enzyme-free protocol
Download In Situ HCR v3.0 Protocolsis used independent of the number of target mRNAs (Choi et al., 2018). qHCR: analog mRNA relative quantitation. dHCR: digital mRNA absolute quantitation.
v3.0 protocols (Choi et al., 2018) for diverse organisms can be adapted from the v2.0 zoo paper protocols (Choi et al., 2016) — the Molecular Technologies Team will be happy to provide guidance (support@moleculartechnologies.org).
Download In Situ HCR v2.0 Protocols
v2.0 protocols (Choi et al., 2014) were established for diverse organisms in the zoo paper (Choi et al., 2016).
|