In Situ HCR v3.0 Protocol Summary
![]() In situ HCR v3.0: multiplexed quantitative mRNA imaging in thick autofluorescent samples with
automatic background suppression throughout the protocol (Choi et al., 2018). ![]() Multiplexing timeline: the same two-stage enzyme-free protocol
is used independent of the number of target mRNAs (Choi et al., 2018). qHCR: analog mRNA relative quantitation. dHCR: digital mRNA absolute quantitation. Download HCR Protein:Protein Protocols
Download HCR IF Protocols
Download 10-plex spectral imaging
Download In Situ HCR v3.0 Protocols
v3.0 protocols (Choi et al., 2018) for diverse organisms can be adapted from the v2.0 zoo paper protocols (Choi et al., 2016) — the Molecular Technologies Team will be happy to provide guidance (support@moleculartechnologies.org).
Download In Situ HCR v2.0 Protocols
v2.0 protocols (Choi et al., 2014) were established for diverse organisms in the zoo paper (Choi et al., 2016).
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