HCR hairpin
In Situ HCR v3.0 Protocol Summary
Insituhcrv3 protocol
In situ HCR v3.0: multiplexed quantitative mRNA imaging in thick autofluorescent samples with
automatic background suppression throughout the protocol (Choi et al., 2018).
Insituhcrv3 multiplexing
Multiplexing timeline: the same two-stage enzyme-free protocol
is used independent of the number of target mRNAs (Choi et al., 2018).
qHCR: analog mRNA relative quantitation. dHCR: digital mRNA absolute quantitation.


Download HCR Protein:Protein Protocols

mammalian cells on slide FFPE human tissue sections


Download HCR IF Protocols

FFPE human tissue sections mammalian cells on slide
generic sample in solution generic sample on slide


Download 10-plex spectral imaging

10-plex spectral imaging


Download In Situ HCR v3.0 Protocols

bacteria in suspension FFPE human tissue sections
generic sample in solution generic sample on slide
mammalian cells in suspension mammalian cells on slide
whole-mount chicken embryos whole-mount zebrafish embryos and larvae

v3.0 protocols (Choi et al., 2018) for diverse organisms can be adapted from the v2.0 zoo paper protocols (Choi et al., 2016) — the Molecular Technologies Team will be happy to provide guidance (support@moleculartechnologies.org).


Download In Situ HCR v2.0 Protocols

bacteria in suspension FFPE human tissue sections
generic sample in solution whole-mount chicken embryos
generic sample on slide whole-mount mouse embryos
whole-mount fruit fly embryos whole-mount sea urchin embryos
whole-mount worm larvae whole-mount zebrafish embryos and larvae

v2.0 protocols (Choi et al., 2014) were established for diverse organisms in the zoo paper (Choi et al., 2016).