HCR hairpin
In Situ HCR Protocol Summary
Insituhcr protocol
Two-stage protocol. Detection stage: probe sets are hybridized to mRNA targets prior to washing unused probes from the sample. Amplification stage: initiators trigger self-assembly of tethered fluorescent amplification polymers prior to washing unused hairpins from the sample.
Insituhcr timeline
Timeline for multiplexed experiment. The same two-stage protocol is used independent of the number of target mRNAs.
Download In Situ HCR v2.0 Protocols

bacteria in suspension FFPE human tissue sections
generic sample in solution whole-mount chicken embryos
generic sample on slide whole-mount mouse embryos
whole-mount fruit fly embryos whole-mount sea urchin embryos
whole-mount worm larvae whole-mount zebrafish embryos and larvae