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In Situ HCR v3.0 Protocol Summary
In situ HCR v3.0: multiplexed quantitative mRNA imaging in thick autofluorescent samples with automatic background suppression throughout the protocol ( Choi et al., 2018).
Multiplexing timeline: the same two-stage enzyme-free protocol is used independent of the number of target mRNAs ( Choi et al., 2018). qHCR: analog mRNA relative quantitation. dHCR: digital mRNA absolute quantitation.
Download HCR Protein:Protein Protocols
Download HCR IF Protocols
Download 10-plex spectral imaging
Download In Situ HCR v3.0 Protocols
Download In Situ HCR v2.0 Protocols
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