HCR hairpin
HCR PPI
HCR protein:protein imaging (PPI):
  • Multiplex
  • Quantitative
  • High-resolution
  • Enzyme-free
  • Automatic background suppression throughout the protocol
  • High signal-to-background in highly autofluorescent samples
  • Compatible with HCR RNA-FISH and HCR IF

HCR PPI: a proximity probe colocalizes a full HCR initiator I1 to trigger HCR signal amplification only if split-initiator probes (P1, P2) are bound to proximal
target proteins (Schulte et al., 2024a).



Simultaneous HCR imaging of RNA, protein, and protein:protein complex targets in HeLa cells (Schulte et al., 2024a).